Wheat Doubled Haploid Facility

The production of homozygous wheat is time and labor intensive, but is a very critical step in wheat research and breeding.  Doubled haploid technology can cut this time down to 2 breeding cycles; reducing the time required for varietal development from 10 to 12 years to 6-7 years.

Doubled haploids are developed via wheat by maize crosses through:

  • haploid induction by pollinating wheat with corn pollen
  • haploid embryo rescue and tissue culture
  • doubling with colchicine to produce fertile homozygous plantlets
  • subsequent seed set

Or through microspore culture:

  • isolation, pretreatment and induction of microspores to form haploid embryos
  • in vitro embryo regeneration
  • doubling with colchicine to produce fertile homozygous plantlets
  • subsequent seed set

Wheat Doubled Haploid Facility is housed in Johnson Hall, room 302 and is operated by WSU  Department of Crop and Soil Sciences. We provide service to WSU scientists and are open to other breeders and researchers.

 

Pricing

  • Production of Doubled Haploid lines in wheat
    • On campus requests – $15 per doubled haploid plant
    • Off campus requests – $25 per doubled haploid plant

Time Frame

  • Production of Doubled Haploid lines from seeds to seeds
    • Winter wheat – 16 month
    • Spring wheat – 12 month

Request Form

  • Download here (docx)

Contact:

Wheat Doubled Haploid Facility
Washington State University
Johnson Hall 302
Pullman, WA  99164-6420 
Phone: 509-335-0044
E-mail: Anna Kondratiuk anna.kondratiuk@wsu.edu